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The concentration of each template was determined fluorometrically (Turner Biosytems, UK).
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This Del-E mutation probe was allowed to hybridize to the RT-PCR products following amplification and its temperature of dissociation (Tm) from each viral template was determined using the LightCycler melting peak analysis.
The cycle threshold (Ct) for each cDNA template was determined on the ABI Prism 7700 Sequence Detection System.
For each sample, the relative amount of starting template was determined by calculating the ΔΔCt after correcting the Ct values for expression of RPL4.
After the best template was determined, fold consistency within the template candidates was considered using TM-score and SCOP database to select additional template structures among the template library.
Through providing the various driving potentials from −25 V, −10 V, −5 V to −2.5 V, the different mechanism of electrophoretically depositing ZnO nanoparticles into the colloidal crystal template was determined by the SEM observation of the filled templates.
The structure of the ternary Dpo4·DNA·dATP complex 4 involving the 5′-TXG-3′ template was determined at 2.75 Å resolution.
The structure of the ternary Dpo4·DNA·dATP complex 2 involving the 5′-TXG-3′ template was determined at 2.9 Å.
The structure of the ternary Dpo4·DNA·dGTP complex 1 involving the 5′-CXG-3′ template was determined at 2.65 Å.
The structure of the ternary Dpo4·DNA·dGTP complex 3 with the 5′-CXG-3′ template was determined at 2.3 Å resolution.
The number of ng amplified by the allele specific primers in the presence of the target template was determined by referencing a standard curve presented in Figure 2. The number of amol was calculated from the ng of DNA derived from the standard curve and corresponding length of coding region (Table 1).
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