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Each template was analysed in triplicate.
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Triplicate or quadruplicate samples of each template were analysed.
Each sample of DNA template was analysed in triplicate for both Beta-globin and HPV16 DNA sequence for 40 cycles.
The nucleosomal composition of this template was analysed by MNase digestion, which produced a DNA ladder upon digestion of poly-nucleosomal DNA.
Standards, samples and negative controls (no template) were analysed in triplicate.
Sixty replicates of diluted template were analysed per sample.
All DNA templates were analysed in duplicates.
Proteins bound to the immobilized templates were analysed by SDS PAGE and detected by western blot using Pierce ECL kits.
In addition, ddH2O as the non-template control was analysed for every plate.
Each sample was analysed in triplicate and a negative control (no template reaction) was included in each experiment, to check out any possible contamination.
Each sample was analysed in quadruplicate.
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