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Each reaction was performed in triplicate for each template using the mean value of the wells for final calculation.
Partial volume estimates and binary-segmented images were also created for each template using the FSL FAST (Zhang et al., 2001) segmentation method.
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The standard 5′ end of the COI region was amplified for each individual DNA template using the primers LCO1490 and HCO219827.
For sample GM03756A, we amplified 1 ng of genomic DNA as template using the MyFi Taq (Bioline).
This is a HOWTO project template, using the linuxdoc dtd.
Each template uses information from all voxels in the brain and represents a spatial pattern present in the reference dataset, similar to a component derived from ICA (in fact, ICA components could be used as templates in a TBR analysis).
Glue these onto the whole egg template, using one as the top and one as the bottom.
The fit will give the best template using discrete parameters of the template model.
In the second normalization step using the software "cg_optimized" [ 6] we locally deformed each image to the new template using a non-linear spatial transformation.
The RMSD of the TMH backbone atoms was calculated for each pair of template structures using the McLachlan algorithm [69] as implemented in the program ProFit (Martin, A.C.R., http://www.bioinf.org.uk/software/profit/).uk/software/profit/
cDNA is synthesized from mRNA template using primers homologous to the immunoglobulin constant-region genes.
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