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Once all solutions were completely aspirated, we applied 1 mL of rinse solution to each strip for 1 minute.
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"Top Chef" host Padma Lakshmi, E! talk show host Chelsea Handler and "Dollhouse" star Eliza Dushku each stripped for the May issue of Allure, the Nude Issue.
Be sure to overlap each strip of tape for full coverage.
Radial profiles of MFA were determined for each strip from the standard deviation of the 002 azimuthal diffraction pattern using the SilviScan-2 instrument (Evans 1999; Evans et al. 1999) located at CSIRO, Melbourne, Australia.
Hold each strip firmly for ten seconds.
In Woolworth's, in mid-April, 1932, White admires the gardening, hunting, and fishing gear that's newly for sale ("pork rind in glass jars, each strip ready punctured for the hook").
One-half of each strip was harvested for whole plant corn silage and the remainder was allowed to mature and harvested as grain.
One milliliter of stringent wash solution was then added to each strip and incubated for 15 minutes at 45°C.
The rinse solution was then completely removed and 1 mL of diluted conjugate was added to each strip, and incubated for 30 minutes.
Following IEF, each strip was equilibrated for 12 minutes in 10 mL of SDS equilibration buffer (50 mM Tris-HCl, 6 M urea, 30% (v/v) glycerol, 2% (w/v) SDS and 0.002% bromophenol blue) containing 1% (w/v) dithiothreitol.
Finally, the reaction was started by adding 100 μL of the tetramethylbenzidine (TMB) substrate (x) every 15 sec to each strip and incubated for another 20 min, and stopped by the addition of citric acid (x) every 15 sec to each strip (and mixed between).
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