An azide reaction scheme has enabled covalent immobilization onto fluorinated ethylene propylene copolymer but its chemistry is less predictable, requiring detailed characterization by XPS and tapping mode AFM after each step of the immobilization sequence.
At each step of the attack sequence, the next sensor to be tampered with is sensor s, where s maximizes E[G s)|I s)], the expectation of the key information gain G(s) given the information I(s) that the attacker knows on sensor s's key ring.
Representativeness of the USD mimics was assessed in parallel by using the pilot scale feed stream generated at each step of the process sequence.
To take into account behavioural constraints related to each step of the B. germanica mating sequence (described below), males and females were tested in different set-ups.
Various problems which may occur in each step of the sample preparation and sequencing can be detected by an analysis of the variation of clusters density; base-call proportions; and base quality across cycles of the run, and across tiles and lanes of the flow cell.
Diversity of GH11 sequences at each step of the capture protocol was evaluated by high-throughput sequencing of GH11 PCR products obtained, as described above, using primers GH11-F and GH11-R.
To evaluate the diversity of GH11 sequences at each step of the capture protocol, we performed a high-throughput Illumina MiSeq sequencing of GH11 amplicons obtained from all four cDNA samples, prior (H0) and after one (H1) or two (H2) cycles of SHS capture.
Then we estimated the allelic bias at each step of the dilution (in multiplex) using a separate sequencing lane.
Enrichment in GH11 sequences at each step of the capture protocol was evaluated by semi-quantitative PCR using different quantities of cDNAs and GH11-fungal-specific degenerate primers GH11-F (GGVAAGGGITGGAAYCNNGG) and GH11-R (TGKCGRACIGACCARTAYTG) amplifying a ±281-bp fragment (Luis P. et al., unpublished).
