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For each stage, approximately 300 larvae were placed in RNAlater (Qiagen) and shipped to Duke University for RNA preparation and sequencing.
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A stratified random sample of 10 faculty members from each career stage (approximately 20% overall) was then drawn from each site.
Banifatemi began her description of the jurisdictional aspect of the case by laying out the timeline, explaining that each stage took approximately five years, with a hearing at the end of each of the five-year periods.
Embryos in broods (one brood consists of 20 40 individuals) from each species of mbuna and non-mbuna were treated during neurula stage (approximately 36 40 h post fertilization) for 5 h at 28 °C, and bathed with either a chemical or DMSO vehicle controls (0.125% for LiCl; 1% for cyclopamine, SAG and IWR).
Also popular with both ice tossers and drink sippers were The Retro Kidz, who brought their asymmetrical hairdos and acid-wash jeans to the stage approximately twenty-seven times.
During the return-stroke stage, approximately 105 joules of energy per metre are dissipated within the lightning channel.
Results show that contamination levels can be modulated by managing the harvesting time and by leaching, with EDTA being effective at early growth stage (approximately ≤ day 6) and acidified methanol effective afterwards.
In the isointense stage (approximately 6 8 months of age), WM and GM exhibit similar levels of intensity in both T1 and T2 MR images, making the tissue segmentation very challenging.
Once the resin has just passed the gel stage (approximately 15-20 minutes after completing the job), cut excess resin away and remove the tape.
Fertilized Atlantic cod eggs (morula stage), approximately 36 48 h post-fertilization, were kindly provided by a commercial cod hatchery in Bodø, Norway.
Preliminary flight data indicated that an anomaly occurred in the rocket's third stage approximately 490 seconds after lift-off, at an altitude of about 160km.
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CEO of Professional Science Editing for Scientists @ prosciediting.com