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The ultimate loads and the failure modes of each specimen were obtained.
The coordinates of the landmarks of each specimen were obtained using the tpsDig software package [42].
Then, two tissue cores from the selected tumor area of each specimen were obtained and included in the TMA.
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Each specimen was obtained with written informed consent from each patient involved.
A measure of laterality for each specimen was obtained by taking the difference between the left and right angles (L-R as in [ 19]).
In addition to the sequence of the PCR band, the exact sequence of one allele for each specimen was obtained by cloning the sequenced PCR product in a plasmid vector.
A focused clinical history and either a blood or saliva specimen were obtained from each patient.
Two rectal/stool swabs or a stool specimen were obtained.
Table 1 provides a complete list of the clinical cases from which each tissue specimen was obtained, also including clinical data, classification by the pathologist and percent of cancer cells present in the specimen, expressed as a mean value between the pre- and the post-RNA sections (see Figure 1).
Each clinical specimen was obtained after subjects signed a written informed consent.
The status of KRAS mutation on codon 12, 13 and 61 was examined by sequencing the KRAS exons 2 and 3. Initially, an H&E staining from each tumor specimen was obtained and reviewed to accurately select tumor area.
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