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The degree of pore connectivity and percolation of each specimen were analyzed in detail on the basis of cluster-labeling algorithm.
Frozen aliquots of each specimen were analyzed for the above viruses plus HMPV by using reverse transcription polymerase chain reaction (RT-PCR) assays and GeneScan deoxyribonucleic acid fragment analysis of PCR products.
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The ROI of each specimen was analyzed for bone volume (BV) and bone mineral density (BMD).
The ROI of each specimen was analyzed for bone volume (BV).
The grain orientation of each specimen was analyzed by an electron back-scattered diffraction (EBSD) method.
A sample of 100 μL of the supernatant from each specimen was analyzed in a UV-Visible spectrophotometer to determine the amount of methylene blue leakage.
Each specimen was analyzed three-dimensionally with a z-axis interval of 0.60 0.64 µm under conventional confocal laser-microscopy (LSM510META, Carl Zeiss, Oberkochen, Germany) equipped with a 40× objective unless otherwise noted.
Each specimen was analyzed for MDA and creatinine.
Each specimen was analyzed for CA 15-3, CEandand TPA.
Each specimen was analyzed by either method at least twice.
Each specimen was analyzed in duplicate, and mean measurements were reported.
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