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The sampling procedure is to split up each single sample of size ni at a controllable regressor's data point into two portions, the first consisting of the ni−1 observations for initial estimation and the second consisting of the remaining one for overall use in the final estimation in order to define a weighted sample mean based on all sample observations at each data point.
Some specific MicroRNA Assays (Life Technologies) were performed on each single sample (3 replicates) in order to assess the miRNAs' expression at the individual level.
Collectively, around 75.5% of the C. beijerinckii 8052 genome was transcribed in at least one sample, even though the fraction in each single sample was much less.
Each single sample Ct value was logarithmically transformed, calculated by the formula 2−(calibrator sample), and expressed as fold change vs the median of disease-free liver Ct.
Each single sample was at least performed twice in terms of technical or biological replicates under a reproducibility of more than 0.975.
The result achieved for each single sample (i.e. the presence or absence of a virulence factor) was compared with the result achieved by q-PCR.
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Each single-sample estimate relates to Ne in a single generation (or Nb for a particular time period), while a temporal estimate depends on the harmonic mean Ne in the entire interval spanned by the samples.
In each year, a single sample was collected from each child.
During each visit, a single sample of 2 mL venous blood from each child was collected into vacuum collection plain tubes without coagulant (VACUTAINER®) by a qualified nurse at the village dispensary and separated by centrifugation at a local hospital.
Each column denotes a single sample, and each differentially expressed gene is represented in an individual row.
Endophytic fungi and Thanatephorus sp., a typical orchid mycorrhizal fungus, were found each from a single sample, on individuals also displaying ECM fungi.
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