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Much work is still to be done on discovering new nuclei, to study the diffusion of each single nucleus and its quantitative weight over the total number of text units of each single manuscript of the tradition.
It also generates a histogram plot for the D values of each single nucleus.
A segmentation mask, identifying each single nucleus as region of interest was set on the Hoechst image.
95% confidence interval (CI) was also determined for the RCN of each assay for each single nucleus giving RCN = 2 × (1 + E −ΔΔCt ± CI, and the upper and lower bounds were used to call copy numbers for APP exons 3 and 14 such that a CI that overlapped with the modeled RCN range was considered as belonging in that copy number bin (See Table 4 for modeled upper and lower bounds).
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DNA of each sorted single nucleus was amplified by WGA before array CGH was performed.
DNA of each sorted single nucleus was amplified by whole genome amplification (WGA) using a modified version of the protocol described by Navin [ 36].
Using the marker-controlled watershed algorithm with these nuclei as markers, we obtained the cellular mask by separating the segmented cellular regions so that each region contained a single nucleus.
Tetrahymena possess a germline and a soma, each represented by a single nucleus within one cell.
Data were obtained for large numbers of individual cells and the data are either shown in scattergrams where each point represents a single nucleus, or as histograms where the mean and standard error of the mean are shown.
Each pollen fragment encompassing a single nucleus was then transferred into a plate well with 9 μl water (Sigma-Aldrich, St . Louis USA).
The number of nuclei was then reduced by applying a less negative potential (>−700 mV vs. Ag/AgCl) to deposit a single nucleus inside each nanopore.
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