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To further analyze p19-RACK1 intheaction, the C-terminal of p19 was mutated at three positions, S160A, W164A, and D165G, and each single mutation was analyzed by yeast two-hybrid assays.
Each single mutation increased the half-inactivation temperature by 2 3 °C over that of the wild-type enzyme.
Each single mutation was associated with a distinct microsatellite haplotype.
PARSESNP (Project Aligned Related Sequences and Evaluate SNPs, http://www.proweb.org/parsesnp/) was used to illustrate the distribution of mutations within the gene, and to indicate the nature of each single mutation.
Thus, their enzymatic phenotype results from the combination of the effect(s) of each single mutation when heteromeric protein forms are generated.
This simple, but efficient, model allowed us to study the actual biological role of each single mutation, since they are pulled out from the context where they originated.
Similar(46)
Instead, the dominant earliest escape variants in WEAU were each a single mutation away from the transmitted form, suggesting they arose independently in WEAU.
Three different genetically engineered variants, each bearing a single mutation, 126, Ile Thr; 136, Lys Glu; and 275, Ser Pro, respectively, showed partial but not complete recovery of reactivity to mAb 503.
Table S1 of the Supporting Information shows the frequency of each type of single mutation.
Briefly, they constructed 9 genotypes, all from the same initial strain, that each had a single mutation caused by the insertion of one of three Tn 10 mini-transposons carrying different antibiotic-resistance markers.
The authors showed that the introduction of only three genes from a producer strain obtained by chemical mutagenesis, each carrying a single mutation, into the wildtype strain led to a tremendous increase in L-lysine production.
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