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For each single cluster, the genes were more or less evenly distributed from the two QTL-regions.
The involucrin and DAPI intensities of each single cluster (no constraint on cell numbers were applied at this stage) were measured (ImageJ, integrated density measurement) and corrected for background levels.
However, on using k = 4 clusters (Supplemental Figure 3), data patterns emerged that were both consistent among donors within each single cluster and significantly divergent between clusters by cumulative immunoblot intensity scores for the five immunogenic salivary proteins (P < 0.05, Student's t test).
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A slightly lower log probability was found for K = 3 (Table S1), which resulted in an identical clustering by habitat, but without further subdivision of individuals from the 'Deep Slope' habitat (i.e. individuals with a 'HostD1' and 'HostD2' mtDNA genotype form each a single cluster).
Chromosome 2 contains the maximum number of clusters (3) comprising of nine StNAC genes, whereas chromosomes 1, 5, 8 and 10 each contain single cluster.
In summary, most of the North American freshwater fish species analysed here exhibit a similar pattern of genetic diversity at COI, each being a single cluster of tightly related mtDNA sequences distinct from all other species.
Rluc8-tagged OX2 receptors and each of the single cluster mutants displayed greater BRET signal stability.
The island pairs Australia/Mayes and Cochons/Cimetière each form a single cluster and Guillou Island forms a clear separate cluster.
Both these variants are expressed in testis, and both are present in approximately 50 copies each in a single cluster on the short arm of the Y.
While each mapped location is assigned to a single cluster, each cluster consists of multiple (at least two) mapped locations.
The considered configurations, instead of assigning each individual to a single cluster, are lists of vectors (one for each individual) carrying their probability to belong to each cluster.
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