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The number of raw fragment within each peak in each single cell were counted using ChromVAR29.
The fragment density was determined by computing CPM (counts per million) values of each peak at each single cell.
scCAT-seq employs a mild lysis approach and a physical dissociation strategy to separate the nucleus and cytoplasm of each single cell.
The number of read within each gene in each single cell (GENCODE, v19) were counted using GenomicAlignments package39 with parameters below: mode = "Union", inter.feature = TRUE and singleEnd = FALSE.
Since the voltage shift is quite small (roughly 0.73 0.83 V for each single cell), the efficiency would be higher.
The striking difference in our cell configuration as compared with constructions reported in the literature is the existence of independent cavities as fuel reservoirs for each single cell.
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This file contains the read depth and alignment rate for each single-cell RNA-seq sample from the Cas9 microinjected controls and the sgRNA2b/Cas9 ribonucleoprotein complex microinjected embryos collected at the blastocyst stage.
Based on the mapped RNAseq reads, gene expression was quantified using Cufflinks 2.2.1 with default settings and treating each single-cell dataset as an individual sample with no replicates while running Cuffdiff (Trapnell et al. 2010, 2013; Roberts et al. 2011a, b).
In each single-cell OFDMA network, K is always much larger than the number of users and no subcarrier can simultaneously support transmission for more than one user.
In addition, some assumptions should be made as follows: (1) In each single-cell OFDMA network, K is always much larger than the number of users and no subcarrier can simultaneously support transmission for more than one user.
Approximately 200 pg of mRNA was injected into each single-cell embryo.
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