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Wear tests were performed for various time duration for each set of specimen.
Average value of the total load at failure (average of one statically loaded and two cyclically loaded) is calculated for each set of specimen (Table 1).
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Long lasting experiments like fatigue test or crack growth test will determine the time required to complete the characterization of each set of specimens.
The quality control was performed by the measurement of customized stored quality control samples along with each set of specimens.
For each set of three specimens, one specimen is tested to know about the failure under monotonic loading, and the other two specimens are tested for cyclic loading (BS 5950: Part 4 [1994]; EN 1994-1-1 [2004]).
Negative (no primary antibody) and positive tissue controls (colon carcinoma) were stained in parallel with each set of tumor specimens studied.
Three different tools were used for each set of ten specimens: a twelve-fluted tungsten carbide bur (123-603-00, Dentaurum, Pforzheim, Germany), a one-step finisher and polisher (inverted cone One gloss, Shofu Dental, Kyoto, Japan) and Adhesive Residue Remover (989-342-60, Dentaurum, Pforzheim, Germany).
For each set of experiments, all specimens in each group of experimental and control tissues were imaged at the same laser intensity and gain settings.
A set of specimen for each externally applied normal load, such that two specimens are utilized for each normal load.
The fact that the second set of specimen though less rough than the first but with a friction angle greater than the first could be an indication of the Evolution law proposed by Zhang and Zhang [4].
In Figure 6, a representative set of specimen was scanned to show the fluorescence distribution representing antibody-stained tubulin which shows an overall increase of fluorescence over the incubation period (Fig. 6).
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