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After six TBS washes of 5 min each, sections were incubated overnight in different antisera.
After washing in PBS three times (3 min each), sections were rinsed with 0.3% Triton X-100 for 10 min followed by washing in PBS.
After washing in PBS thrice (3 min for each), sections were rinsed with 0.3% Triton X-100 for 10 min followed by washing in PBS.
After washing in PBS thrice (3 min for each), sections were treated with 0.04% DAB and 0.03%H2O22 at room temperature for visualization for 8 12 min.
Following two immersions in 100% resin (minimum of 6 hours each), sections were embedded individually in beam capsules and hardened at 60°C over 24 hours.
After five washes in 0.3 % Triton-X-100 in PBS for 15 min each, sections were incubated in secondary antibody overnight at 4 °C.
Similar(47)
At least three selections from each section were inspected.
Operation tests of each section were conducted to confirm basic functions of reactors and separators, etc.
Fiber distribution coefficients and fiber density maps of each section were determined.
Tensile coupons in the flat portions and corners of each section were tested.
The number of plaques in each section were summed and then averaged for each animal.
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CEO of Professional Science Editing for Scientists @ prosciediting.com