Sentence examples for each second round from inspiring English sources

For each second round primer pair, optimization of the conditions for PCR and the subsequent HRM step were carried out, noting that the presence of the LCgreen Plus dye increased the primer Tm and thus raised the optimum annealing temperature of the PCR reaction.

The labelling PCR mastermix consisted of 5 μl 10x AccuTaq™ LA DNA Polymerase buffer (Sigma-Aldrich), 1 μl of each second round PCR primer (Eurofins, 20 pmol/μl), (1 mM of each dATP, dCTP, dGTP, 0.65 mM dTTP and 0.35 mM biotin-16-dUTP, Roche Applied Science), 0.5 μl AccuTaq™ LA DNA Polymerase (Sigma-Aldrich, 5 u/μl), 28.5 μl water and 5 μl template.

They also received their own ratings of each first round attribute in relation to the group distribution.

The number of statements presented in each first round questionnaire differed markedly across topics and was largely determined by the amount of accessible literature on the area.

Therefore, two second-round PCRs were performed for each first round PCR sample, one with primer mix A and the other with mix B in a volume of 20 μl with nested PCR primers.

Almost as striking in that regard is the difference between each second-round bracket's four teams, which might, somewhat paradoxically, explain why the San Diego bracket has been more attractive.

Here is a look at each first-round series: RED BULLS (15-9-6, 51 POINTS) VS.

In the qualifying tournament format, the top two teams from each first-round group advance to the semifinals.

Each first-round loser in the singles main draws earns $23,000 (and a Citizen wristwatch), or $4,000 more than in 2011.

The top two teams in each first-round group advance to the semifinals, with the two finalists advancing to the Olympics.

Tickets for the first round of the playoffs cost roughly 25 percent more than regular-season games, so the team would generate about $2 million in ticket revenue for each first-round home game.