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Significant difference (p < 0.05) of protein measurements between the two groups at each sampling was determined by calculation of the 95% confidence interval for the difference in population medians.
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The protein concentration of each sample was determined with a Micro BCA Protein Assay Kit (ThermoScientific).
The Mn concentration for each sample was determined by triplicate measurements.
The supernatant was carefully removed, and concentrations of each sample was determined using a bicinchoninic acid (BCA) assay.
The concentration of each sample was determined with UV spectroscopy by using the standard curve method (λmax, 484 nm).
Finally, absorbance values were calculated for standards and samples and the concentration of each sample was determined using the standard curve.
Absorbance for each sample was determined three times.
Peak area of each sample was determined using the HP 3365 series II ChemStation software.
As the first step, the mass of each sample was determined.
Seed flavonoid content of each sample was determined following colorimetric method (Chang et al. 2002).
The thermal conductivity, effective porosity, permeability, and matrix density of each sample was determined.
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