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On each sampling occasion the environmental variables were measured.
The model controlled for farm and cohort by a nested design and included a repeated measure on calf at each sampling occasion.
Measurements were made on fully expanded sun leaves (10 leaves per species per each sampling occasion) during the study period.
At each sampling occasion, a total of 16 samples were collected, packed in 10-L buckets and transported to the laboratory.
On each sampling occasion, leaf respiration rate [RD, μmol (CO2) m-2 s-1] measurements were carried out contemporary to PN ones (on the same leaves) by darkening the leaf chamber with a black paper, according to Cai et al. (2005), for 30 min prior to each measurement to avoid transient post-illumination bursts of CO2 releasing (Atkin et al. 1998a, 1998b).
About 70% of the data came from newly established random quadrats at each sampling occasion.
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Written consent from each dog owner was collected by the veterinarian on each sample occasion using a specific consent form approved by the Ethical committee.
After each completed sampling occasion, the farmers were sent information on their herd's serological status and information about basic biosecurity measures.
On each subsequent sampling occasion marked individuals can be relocated and their mark recorded, and unmarked individuals may be marked for the first time.
One CoNS isolate of each colony-morphology type, from each site and sampling occasion, was stored in glycerin-containing broth at -70°C until further analysis.
Four out of five (80.0%) of the kittens enrolled in T03 and which had received no treatment, were observed to be positive for FeLV p27 antigen on each post challenge sampling occasion and therefore these four animals each met the criteria for persistent antigenaemia.
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