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Three points of each sample were tested, and the average value of the three left and right contact angles was calculated as the determined static contact angle [10, 11].
Duplicates for each sample were tested in three dilutions and the mean values of the duplicates from the optimal dilution were reported.
Two technical replicates for each sample were tested.
Finally, 40 μL of homogenate of each sample were tested in triplicate using the 30-plex gene panel.
Deviations from linkage disequilibrium (LD) between all pairs of loci for each sample were tested using arlequin.
Duplicates for each sample were tested in three dilutions and the mean values of the duplicates from the optimal dilution were reported [ 28].
Similar(51)
Each sample was tested in duplicate or triplicate.
Each sample was tested in triplicate for SYBR Green assays, and in duplicate for Taq Man assays.
Each sample was tested in triplicate: 30 μL (1), 20 μL (2), 10 μL (3) of cell free lysate, respectively.
Each sample was tested in three to six laboratories using a variety of commercially available or laboratory-developed platforms.
Each sample was tested with the same ELISA as used for BTM testing, and cows were considered test positive for S/P values ≥40, and otherwise negative.
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