Sentence examples for each sample were quantitated from inspiring English sources

To determine relative protein amounts, three representative exposures for each sample were quantitated by densitometry analysis using the ImageJ free software.

The proteins from each sample are quantitated using Bradford or BCA reagents and compared with bovine serum albumin (BSA) standards for accurate protein estimation.

The protein concentration of each sample was quantitated by using a Dc Protein Assay Kit (Bio-Rad; Hercules, CA), and equal amounts of protein for each sample were separated by SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad), blocked with 10% skim milk, incubated with specific antibodies, and visualized using a Super Signal West Pico Chemiluminescent kit (Pierce; Rockford, IL).

An aliquout of each labled sample was quantitated by liquid scintillation counting using a Beckman LS 6500.

The standard contains 0.01 mg/mL of amiodarone, and USP specified impurities D and E with a resolution requirement of NLT 3.5 between peaks D and E. Impurities in a 5 mg/mL sample are quantitated against the standard.

After PCR, the sample was quantitated with the Invitrogen Qubit fluorometer.

HSP 70 concentrations from the sample are quantitated by interpolating absorbance reading from a standard curve generated with the calibrated HSP 70 protein standard provided.

In one of these, the radioactivity of the sample was quantitated by scanning laser densitometry (Desk TopTM Scanner Plus, Amersham Pharmacia Biotech).

Briefly, 20 ng gDNA for every sample was quantitated by Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA) for multiplex PCR amplification with the 2X primer pools.

Total RNA samples were quantitated using the fluorescent Ribogreen RNA quantitation reagent kit (Molecular probes) and RNA was stored at −70°C until use for RT PCR.

All samples were quantitated using the comparative CT method for relative quantitation of gene expression, normalized to β-actin and 18S [ 24, 25].