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About 100 spores from each sample were picked up under the stereo microscope and placed in 1.5-ml tubes for DNA extraction.
For 3D classification of apo-hDicer and hDicer-RNA complexes, about 70,000 particles of each sample were picked together from each set of micrographs.
Eight single colonies of each sample were picked and re-cultured on a new LB plate; three of these single colony clones were sequenced to determine the allelic forms of S1, S3, S5 and S24, and the other five served as back-ups.
At least 8 clones from each sample were picked for sequencing using BigDye sequencing kit and analyzed on an ABI 377 DNA Sequencer (Applied Biosystems, Foster City, CA).
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Five clones of each PCR sample were picked up and sequenced.
50 embryos per sample were picked from grape plates, transferred into lysis buffer, supplied by the Absolutely RNA Miniprep Kit (Stratagene), and immediately frozen on dry ice to break the chorion.
The World Anti-Doping Agencodeode, for instance, requires a rigorous record of each time the sample is picked up and set down -- by whom, and when.
Each child's urine sample was picked up from the home by a project staff member the morning the sample was collected, and transported in a cooler with blue ice to our field laboratory, where it was frozen to –20°C.
Four biological replicates were grown from each species and three samples were picked from each biological replicate.
Polls inevitably include a margin of error, because they draw on subjective opinions; data from a sample of graduates, however the sample is picked, is not perfectly representative of all graduates; and the measures captured by each ranking are only imperfect proxies for performance, not performance itself.
If the target slab is deemed suitable, a number of test holes are likely to be drilled - using the rotation as well the percussive action - before a powdered sample is picked up and delivered to Curiosity's onboard laboratories.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com