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Three different fields in 2 droplets of semen from each sample were examined, giving 6 motility estimates per examiner for each ejaculate.
The chemical composition of the surface and in-depth layers of each sample were examined by X-ray photoelectron spectroscopy and X-ray diffraction measurements.
Four random high-power fields in each sample were examined from three independent experiments.
More than 20 ultrathin sections of each sample were examined with a JEM ×1200 transmission electron microscope (Nikon, Japan).
At least 100 randomly selected elongating spermatids and sperm from each sample were examined for the presence of chromatin vacuoles.
At least 100 randomly selected round spermatids from each sample were examined for the presence of chromocenter fragmentation.
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Dissolved molecular oxygen effect on the enhancement factor for each sample was examined experimentally.
After the assigned annealing time, each sample was examined with TEM to observe its general crystal configuration.
Each sample was examined with the SEM at different magnification to look at the nanowire morphology, growth direction and growth density.
Immediately after debonding, the enamel surface of each sample was examined in a stereoscopic magnifier (Bel Microimage Analyser Bel Photonicss, Monza, Italy) with 10× magnification, to determine the amount of adhesive remaining.
Each sample was examined for its appearance and for its physical and chemical properties using scanning electron microscopy (SEM) (Zorn, 2014).
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