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At predetermined time intervals, 2 mL of each sample was withdrawn and immediately replaced with an equal volume of fresh PBS (pH 6.0).
This low pH ensured a high recovery of the amphoteric OC. 700 ng of the deuterated internal standard OCD3 was added to each sample and 200 mL aliquot of each sample was withdrawn and subjected to extraction.
100 μg of each sample was withdrawn and diluted to 200 μl.
Immediately, 20 μl of each sample was withdrawn and assayed for GST activity.
Similar(56)
In all cases, aliquots of each sample were withdrawn at different times and assayed for enzymatic activity under the standard condition.
Aliquots of each sample were withdrawn at different times and assayed for sulfoxidation and peroxidase activity under the standard conditions as stated above.
The chamber was replenished with an equal volume fresh medium each time a sample was withdrawn.
At predetermined time intervals (0, 5, 10, 15, 30, 60, 90, and 120 min) an aliquot (5 mL) of the sample was withdrawn from each vessel and immediately replaced with an equal volume of fresh medium to maintain sink conditions.
After 1 h, 1 ml of air sample was withdrawn from each jar for the ethylene measurements.
To determine sporulation efficiencies (which were usually ∼80%), a sample was withdrawn from each culture 24 hr after suspension in SPM and examined by light microscopy.
Each time 5 mL of the sample was withdrawn from the vessel at suitable time intervals and an equal volume of the dissolution medium was added in order to maintain the sink condition.
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