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Each sample was viewed at 2000× magnifications.
Larval remains were macerated and combined with an equal volume of ice cold 3% sodium citrate (pH 8.4) containing 5 μg/ml actinomycin D, and stored at −80° C. A small amount of the material from each sample was viewed under a microscope to confirm the presence of A. apis ascomata, and any sample lacking such spore cysts was discarded.
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A minimum of five eggs from each sample were viewed using a Zeiss Stemi 2000-C stereo microscope under varying magnification (10 50×), and digital images were taken with a Megapixel FireWire digital camera attached to the microscope.
The sample was viewed under TEM (Hitachi H-7100).
The sample was viewed under a fluorescence microscope using a 495 nm excitation filter and a 515 nm barrier filter.
The sample was viewed 48 hours later under a fluorescent microscope, where successfully transfected cells expressed the red fluorescent protein as shown in Fig. 6.
Any participants who did not take the second sample within 20 40 min of the awakening sample were viewed as non-compliant.
There are different ways to identify the components, depending on how the sample is viewed at its various levels of molecular and structural granularities.
Shared OTUs (at genus level) between different samples was viewed using online tool Venny (http://www.bioinfogp.cnb.csic.es/tools/venny/index.html).html
The microstructure of the samples was viewed via JEOL, JSM-6390LA Scanning Electron Microscope (SEM) for observation of starch granules and changes occurring on cluster of sago hampas.
At least five samples were viewed from each stage of tissue culture and tissue culture condition.
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