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Transfection efficiency of each sample was verified through GFP expression and analyzed concurrently by FACS.
Efficiency of labeling from each sample was verified by mass spectrometry before the eight samples were pooled.
Each sample was verified visually by examining the PCR curves generated to eliminate false positives due to aberrant light emission.
The quality of each sample was verified by running an aliquot on a Bioanalyzer (Agilent Technologies, CA).
For RNA-Seq, the RIN of each sample was verified to be nine or more using an Agilent 2100 Bioanalyzer, and ∼4 µg total RNA per sample were processed using the TruSeq Kit (as noted above) to produce indexed libraries.
The integrity of the RNA for each sample was verified by RT PCR with primers for human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), as described elsewhere (Osella-Abate et al, 2000).
Similar(53)
Twelve clones of each sample were verified by sequencing with the T7 universal primer.
Purity and concentration of RNA of each sample were verified at 260 nm.
The integrity of each RNA sample was verified by performing RT PCR for porphobilinogen deaminase (PBGD).
The amount and quality of each RNA sample was verified on an Agilent 2100 Bioanalyser.
Total RNA concentration was determined measuring OD260, and the integrity of each RNA sample was verified using an Agilent 2100 Bioanalyzer (Santa Clara, CA).
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