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The concentration of each sample was validated using the Nanodrop spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).
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Field and museum identifications for each sample were validated by amplifying and sequencing a portion of the mitochondrial cytochrome b (Cytb) gene using the methods of Stadelmann et al. (2007).
The enriched target DNA in each library sample was validated and quantified by microfluidics analysis using the Bioanalyzer High Sensitivity DNA Assay kit (Agilent Technologies) and the 2100 Bioanalyzer with the 2100 Expert Software.
HSP_NumtS not present in the European sample were validated in an Ethiopian sample whose mtDNA belonged to the L0 haplogroup.
The method developed for analyzing the detoxified samples was validated as per ICH guidelines Q2R1.
Additionally, a method for estimating the tracer input function from the tracer brain tissue kinetics and venous sampling was validated.
The online SPE LC/MS/MS method for waters samples was validated in terms of specificity, linearity, limit of detection, matrix recoveries and inter-day precision of the technique.
After optimisation, application on environmental water samples was validated, and specificity towards the targeted virus types was obtained through the qPCR step.
Differential expression of these genes in either Cr_large or Cr_small samples was validated using quantitative real-time PCR.
FOX gene promoter hypermethylation in patient samples was validated by bisulfite sequencing analysis (BSA).
This method of biopsy sampling was validated by the histological and immunohistochemical analyses.
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