Sentence examples for each sample was reversed from inspiring English sources

For mRNA qRT-PCR, 2 µg of total RNA from each sample was reversed transcribed with Superscript III (Invitrogen).

2.4 μg total-RNA of each sample was reversed transcribed to cDNA using Superscript II (Amersham, Buckinghamshire, UK) and random hexamer primers, all according to supplier's protocol.

To test the expression of Hes-1 in the transfected cells, 2  μg of total RNA extracted from each sample was reversed transcribed into first-strand cDNA by RevertAid First Strand cDNA Synthesis Kit (Fermentas), according to the manufacturer's instructions.

To prepare the real-time PCR assay, 1 µg of total RNA from each sample was reversed transcribed using the SuperScript™ III First-Strand Synthesis System for RT-PCR (Invitrogen Inc). in the presence of random hexamers.

Dyes used to label each sample were reversed in half of the replicates to control for dye-by-gene interactions.

Five micrograms of total RNA from each sample were reversed transcribed with Superscript II (Invitrogen) following the manufacturer's instructions.

Five micrograms of total RNA from each sample were reversed transcribed with Superscript (Invitrogen) following the manufacturer's instructions.

Subsequently, 2 µg RNA from each sample was reverse transcribed using 'PROMEGA RT kit'.

RNA (1 mg) from each sample was reverse-transcribed using 25 U of superscript II reverse transcriptase (Invitrogen).

A total of 2 µg RNA from each sample was reverse transcribed into cDNA using the protocol described above.

One µg of total RNA from each sample was reverse transcribed with a Retroscript RT kit (Ambion).