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Twenty percent of each sample was retained as a 'Total' fraction, and precipitated in 4 volumes of methanol.
A 5 µL aliquot of each sample was retained to measure total bisulfite treated DNA using the real-time PCR assay (HB14) described in Supplementary Table S1.
A 5 µL aliquot of each sample was retained to measure total genomic DNA using the real-time PCR assay (CFF1) described in Supplementary Table S1.
Approximately 40 μl of each sample was retained as 'total input'.
At this stage a 50 μl aliquot of each sample was retained and boiled in Laemmli electrophoresis buffer [ 14] as input controls.
Similar(55)
reads for each sample were retained and used for further analysis.
Only alignments with a single copy from each sample were retained, and several of these exons were partial.
The first 3,000 samples were discarded (burn in), and of the remaining samples, every tenth sample was retained (thinning).
Characterization by transmission electron microscopy (TEM) revealed that the microstructure of the shock-consolidated sample was retained at the nanoscale.
If the average distance of a test sample was lesser than or equal to the threshold value, the test sample was retained within the AD.
The other half of the sample was retained as an unexposed control.
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CEO of Professional Science Editing for Scientists @ prosciediting.com