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Each sample was resolved by SDS-PAGE on 20% polyacrylamide gels and then transferred to 0.45-μm nitrocellulose membranes.
Equal amount of the proteins (50 μg) of each sample was resolved on SDS-PAGE and then electro transferred to PVDF membrane (Millipore Corp .. Nonspecific binding was blocked by incubation with 5%% nonfat milk in Tris-buffered saline containing 0.1%Tween-20Tween-20) for 1 h at room temperature.
Each sample was resolved by 7.5% polyacrylamide gel electrophoresis and immunoblotting with appropriate antibodies as indicated.
Each sample was resolved for 10 min at a flow rate of 0.5 ml/min.
An aliquot of each sample was resolved by SDS-PAGE and transferred using standard semi-dry conditions.
40 µg of lysate for each sample was resolved on 10% polyacrylamide gels (SDS-PAGE) under denaturing conditions and then electrophoretically transferred to 0.45 µm nitrocellulose membranes.
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After centrifugation 20 µl of each sample were resolved by 10% SDS-PAGE.
Equal amounts of protein from each sample were resolved by PAGE and analyzed by western blot.
Proteins (15 µl of each sample) were resolved with 12% SDS-PAGE, followed by transfer to nitrocellulose.
Forty micrograms of total protein extract from each sample were resolved by 10% SDS-PAGE and transferred to nitrocellulose membranes (Amersham Biosciences).
The samples were harvested in 2× sample buffer (125 mM Tris (pH 6.8), 4% sodium dodecyl sulfate (SDS), 0.01% bromophenol blue, 10% β-mercaptoethanol, 10% glycerol) and equal amounts of protein from each sample were resolved by 10% SDS-PAGE.
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