Sentence examples for each sample was removed from inspiring English sources
After the removal of the insoluble material by centrifugation, 30 µl of each sample was removed and stored (input), while the rest was diluted in immunoprecipitation buffer (1% Triton X-100, 2 mM EDTA, 150 mM NaCl and 20 mM Tris/HCl, pH 8.1).
The outer edge of each sample was removed to minimize the variability caused by the un-patterned sidewalls by using a sterile 8 mm biopsy punch (VWR International); the 8 mm samples were then aseptically loaded into a 15 mL conical tube containing 2 mL sterile Dey-Engley broth (Sigma Aldrich).
10 µL of each sample was removed and reserved as "Catch-2 aptamer-bound eluate" for SDS-PAGE analysis.
At the end of the various incubation times (0, 6, 12, 18 and 24 h), each sample was removed from the medium and washed with PBS.
The samples were spun out of the plate by centrifugation at 1000 RCF for 30 seconds. 10 µL of each sample was removed and reserved as "Catch-1 eluate" for SDS-PAGE analysis.
After protein isolation, 100 μg from each sample was removed for the PKB/Akt activity assay.
Three 100 l aliquots from each sample were removed and added to 1 ml Biofluor scintillation fluid (Perkin Elmer, Cambridge, UK).
The pieces of each sample were removed, transferred to 2.5% glutaraldehyde in sodium cacodylate buffer 0.1 M for transmission electronic microscopy analyses or to Zamboni fixative solution [41] for light microscopy.
Slices of each sample were removed and homogenized in 1 mL of buffer before centrifuging at 3000 ×g for 10 min.
For this purpose, the testes from three animals chosen randomly on each sampling were removed, washed in PBS (pH 8.2), macroscopically observed for the degree of dispersion of the cell suspension (see Figure S3), and examined under a fluorescent microscope (Nikon Eclipse E600, Tokyo, Japan) for the presence of CFDA-SE-positive donor cells.
Each culture sample was removed from the incubator for about 60 minutes, and each individual neuron test lasted about 40 minutes.
