Sentence examples for each sample was quantified from inspiring English sources
Each sample was quantified by determining the cycle threshold (Ct) and triplicate qRT-PCR reactions were done for each mRNA samples.
The sp3 C fraction of each sample was quantified by using electron energy-loss spectroscopy (EELS).
The DNA concentration of each sample was quantified using Qubit dsDNA BR Kit according to the manufacturer's instructions (Thermo Fisher Scientific).
Following DNA extraction, the mtDNA copy number in each sample was quantified by real-time polymerase chain reaction (PCR).
To explore the role of low parasitaemia in apparently negative pfhrp2 /pfhrp3 genotyping results, the parasitaemia of each sample was quantified using a previously published PgMET qPCR assay, which has a limit of detection of 5 parasites per µL31,32.
Samples were exposed to bacteria in solid media (nutrient agar), and the inhibition zone around each sample was quantified and put down as the antibacterial effect.
Protein in each sample was quantified using the BCA method.
A specific standard curve was performed in parallel for each gene, and each sample was quantified in duplicate.
Initially, the total RNA for each sample was quantified using a DU 530 UV/VIS spectrophotometer (Beckman Coulter, Brea, CA).
Each sample was quantified by spectrophotometry (Nanodrop), quality assessed by agarose gel electrophoresis, and diluted to a concentration of 50 ng/μL in 5 mmol/L Tris, pH 8.5.
