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The samples were then centrifuged at 1900 g for 15 min at 20 ± 5 °C and the serum obtained from each sample was distributed to two fresh polypropylene test tubes in two aliquots of 1 ml each, one for rhNGF determination and the other for anti-NGF antibody detection by ELISA methods following the manufacturer's instructions.
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In Bushbuckridge the sample was distributed proportional to the number of deliveries in each of the two hospitals.
The sample was distributed proportionally according to the number of registered births in each of the chosen facilities during the previous year.
The sample was distributed equally to two 2 mL reaction tubes, if necessary.
The global sample was distributed into 12 strata, in principle, in proportion to the number of pupils in each stratum.
Two aliquots of each RNA sample were distributed to each of three centers: Oregon National Primate Research Center, Yerkes National Primate Research Center and the University of Nebraska Medical Center.
On the 48.48CS dynamic array, each DNA sample is distributed into 48 reaction chambers to genotype 48 SNPs.
Sample were distributed to each of four centers, and one center processed two sets of the 16 samples at different times using different labeling protocols.
Figure 1 shows how the sample is distributed on the neighborhood scale.
Verified SNPs from the Montana sample were distributed among 484 contigs of the combined assembly, and verified SNPs from the Utah sample were distributed among 767 contigs.
Sampling was distributed along the 2-month survey.
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