The Mn concentration for each sample was determined by triplicate measurements.
The protein concentration of each sample was determined with a Micro BCA Protein Assay Kit (ThermoScientific).
The supernatant was carefully removed, and concentrations of each sample was determined using a bicinchoninic acid (BCA) assay.
The concentration of each sample was determined with UV spectroscopy by using the standard curve method (λmax, 484 nm).
Absorbance for each sample was determined three times.
Peak area of each sample was determined using the HP 3365 series II ChemStation software.
As the first step, the mass of each sample was determined.
Seed flavonoid content of each sample was determined following colorimetric method (Chang et al. 2002).
The thermal conductivity, effective porosity, permeability, and matrix density of each sample was determined.
After incubation, the MIC of each sample was determined by visual inspection of the tubes.
Each sample was determined in triplicate.
