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Given the inherent conflict between the desire to collect material in a uniform and optimal manner and the constraints of working in a clinical environment where such collection is not always feasible, the description of how each sample was collected, handled and stored, will be very important.
Each sample was collected from six different, randomly selected plants.
Protein concentration was determined and 10 mg of protein from each sample was collected for further processing.
To analyze the effects of composite on osteoblasts, primary cultures, each sample was collected on days 1, 3 and 7 after seeding.
Each sample was collected from 0-30 cm soil depth.
Finally, each sample was collected at different time points: 2, 4 and 6 days.
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Each sample is collected by an independent module, so failure of a single unit does not affect others.
Results from each sample were collected in triplicate right after sonication.
A total of 20,000 events for each sample were collected and analyzed with the CellQuest Pro software.
Three replicates of each sample were collected.
Twelve fractions from each sample were collected using a Gradient Station.
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