Sentence examples for each sample was clarified from inspiring English sources

Each sample was clarified by centrifuging at 4000xg at 4°C for 10 mins and stored in three aliquots at −80°C in CompleteTM Protease Inhibitor Cocktail (Roche, Mannheim, Germany) until further analysis.

After 30 min of end-to-end rotation in the cold room, sample was clarified at 21,100 × g for 10 min.

The sample was clarified by centrifugation, and 1.5 mL of the supernatant was analyzed for free amino acids.

The final sample was clarified using membrane filters (pore size of 0.45 μm and diameter of 4 mm).

Briefly, 1 L of water sample was clarified by filtration through Whatman 597½ paper (Whatman).

The sample was clarified at 30,000 × g for 15 min prior to injection into an HiPrep Heparin 16/10 column equilibrated in buffer B5 at 1.5 ml/min.

Following sonication, the sample was clarified by centrifugation at 100,000× g for 30 min. One molar imidazole (pH 7) was added to the clarified lysate to a final concentration of 40 µM.

In addition, ionic milling enlarges particle numbers which are thin for electron transmission and also ambiguity of core/shell structured nanoparticle in the conventional TEM sample are clarified as shown in Figure 4c,e,f,e,f

Sample were clarified by centrifugation and loaded on a 7.5% Tris-glycine native gel.

Prior to glycan removal, 100 µL of the SMSL or parotid saliva samples was clarified by centrifugation (3000× g) at 4 °C for 20 min.

Saliva samples were clarified by centrifugation at 16,000 × g for 10 min at 4 °C.