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A set of 3 images from each sample was chosen.
Each sample was chosen to have the same number of sequences and average sequence length with the UCE data set: 481 sequences, 262 bp each.
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The number of iterations should be many times the number of samples to ensure each sample is chosen several times.
At least eight random regions on each sample were chosen to be photographed, and the mean number of live cells was calculated.
In statistics, stratified sampling is obtained by taking samples from each stratum or sub-group of a population, i.e., a mini-reproduction of the population is achieved; conversely, according the random sampling method, each sample is chosen entirely by chance in order to reduce the likelihood of bias.
Six images for each sample were chosen, paying particular attention to select only those in which the section crossed the central plane of the cell.
Two typical spots for each sample were chosen based on the H&E staining.
Three sections from each sample were chosen randomly and observed under LM for histomorphometric analysis.
The TOF spectra were recorded in the positive ion reflector mode with a mass range from 700 to 4000 Da, and 10 of the strongest peaks in each sample were chosen for MS/MS analysis.
A minimum of 5 transcript reads (on average), with at least one read from each replicate sample, was chosen as the cut off point to reliably indicate reproducible expression across biological replicates [ 24].
From each sampling site, one sample was chosen for each leaf age.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com