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Additionally, the surface roughness of each sample was checked after electropolishing with the aid of Atomic Force Microscopy (AFM).
The quality of each sample was checked by a Bioanalyzer 2100 (Agilent, Waldbronn, Germany).
Fatty acids used in biological activity assay could lower the pH of the medium so each sample was checked for the pH value before the experiments.
Gender of each sample was checked with the number of heterozygosities at SNPs on chromosome X and the relatedness of individuals was analysed using the GRR program [71].
Before weighing and lyophilizing, each sample was checked under a binocular microscope to confirm that there was no contamination with other pests (e.g., spider mites or thrips) or leaf pieces.
RNA integrity in each sample was checked by amplifying a β-actin gene segment.
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The taken volume of each sample is checked gravimetrically according to their initial densities by an AND balance with ± 10−4 g resolution (GR-300).
Contigs for each sample were checked and edited using Sequencher 4.8 (GeneCode, Boston, USA).
In the analysis of the sequences obtained, chromatograms of each sample were checked to reduce PCR or sequencing errors.
Raw reads data for each sample were checked using FastQC (version 0.10.0, Babraham Bioinformatics, Cambridge, United Kingdom; http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/) to identify features potentially indicative of quality issues (e.g., low-quality scores, overrepresented sequences, and inappropriate GC content).
The integrity of each RNA sample was checked with an Agilent Bioanalyzer® (Agilent Technologies).
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