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Every sampling was done at morning 10.00 am and samples of nine randomly selected plants (3 from each replication) of each cultivars were pulled together.
Approximately 200 mg each of rachis and hulls were collected from each replication of NT plants, digested, and the metal concentration was measured.
Each replication of water content, amylase activity, desiccation resistance and microarray assays represented an independent germination experiment.
For each replication of protein extraction, apoplast fluid from five tubes was pooled, and five milliliters of Tris-saturated phenol (pH 8.0) were added.
Then a multivariate Cox regression model with backward selection was fitted to each replication of the original data set and for each ability.
Within each replication of the cross-validation, 80% of RILs (3484) of each family were randomly sampled without replacement from the 4354 common RILs to use as a training set.
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Our priority is to maximize the quality of each replication to adequately test the research question.
For each hybridization experiment, one technical replicate (using independent labelling reactions) was performed, each replication consisting of a reverse labelling experiment.
Each replication consisted of one glass jar and all experiments were replicated four times.
Each replication consisted of one spike harvested from the second and third tiller of the plants.
Each replication consisted of 8 rows with 12 plants per row.
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