Each bootstrap replicate involved two replicate heuristic searches with random addition of taxa, saving a maximum of 50 trees per random addition replicate.
Our particular Plackett-Burman (2^7*3/16) type III resolution design with two center points and one replicate involved 14 randomized runs.
Four dye-swap comparisons (2 for each biological replicate, involving a total of 8 microarray slides) were performed for each comparison, for a total of 56 hybridizations in the study.
Technical replicates involved multiple hybridizations of single RNA preparations.
The high-throughput sequencing techniques, even being more informative [ 31, 32], are still not economically affordable for inoculation impact analysis, because of the high number of samples and replicates involved.
Video recordings, skin and tail lesions, lameness scores and performance were collected during three replicates involving 216 pigs each.
Replicates involving the same colonies were separated by more than one week to minimise memory of the previous situation, which is not expressed after 6 days [50].
This cross-validation replicate process involved the random splitting of occurrence data into a number of equal-sized "folds" or groups where models were created leaving out one fold for each run.
Each experiment involved five replicate transfections performed on three separate occasions.
