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(Delta_{r} G_{m}^{theta }) of each reactions are calculated from (Delta_{r} H_{m}^{theta }) and (Delta_{r} S_{m}^{theta }) at the target temperature.
After washing in PBS three times (3 min each), reactions were visualized by treating sections with 0.04% DAB and 0.03% H2O2 at room temperature for 8 12 min.
To identify entry points of pathways, for each reactions of the macrophage model under gene control flux variability analysis was performed to determine in which direction the reaction can carry a flux.
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Each reaction is a setup for another.
Each reaction is catalyzed by a specific enzyme.
To each reaction corresponds a reverse reaction.
Modified Arrhenius equation applied for each reaction.
The energy barrier for each reaction is determined.
The advantages and disadvantages are discussed for each reaction.
Three replicates were carried out for each reaction.
The method is tested for each reaction as follows.
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