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In summary, parallel sections were pretreated with H2O2 in phosphate-buffered saline at 37°C for 15 minutes to eliminate endogenous peroxidase, rinsed twice in 0.05 M Trizma buffered saline (TBS) containing 0.1% Tween-20 at pH 7.4 (TBS-T) for 10 minutes each, pretreated with blocking avidin/biotin kit and then incubated overnight with the primary antibodies as described in Table 1.
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Four groups of five mice each were pretreated with extracts (250 and 500 mg/kg), diclofenac (1 mg/kg) and distilled water (3 mL/kg).
During December 2008, one bale of each cultivar from each site was pretreated in Inbicon's pilot plant [ 19, 20] in separate runs.
The results shown in Figure 5 demonstrated the effects of the pretreatment on the ethanol yield from each of the pretreated samples.
Finally, the third set of animals (n = 10) was divided into two groups (n = 5 each) that were pretreated i.p. (24 h before the start of the experiments) with, respectively: (i) 1 ml/kg of 5% ascorbic acid; and (ii) 5 mg/kg reserpine.
Each subject was pretreated for a week with professional dental hygiene.
Adjacent 50 μm thick sections of each brain were pretreated with citrate buffer for 30 min at 65°C to increase antigen retrieval and penetration of the antibodies into the tissues.
Five groups of mice (5 mice in each group) were pretreated with control, Diclofenac Na (100 mg/kg) and the extract (500, 1000 and 2000 mg/kg).
Cells of each genotype were pretreated with 250 nM BDE-49 or BDE-47 for 16 hr before loading them with the Ca2+-sensitive dye Fluo-4.
The cells in each well were pretreated with 1 mL of live lactobacilli (1 × 10 CFU/mL) or S. typhimurium (1 × 10 CFU/mL) for 2 h.
To explore desensitization directly, we briefly pretreated each RASSL with 1 nM spiradoline for 15 min, and examined inhibition of cAMP accumulation in response to a variety of doses of spiradoline.
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