Sentence examples for each precipitated from inspiring English sources

Ostwald's theory postulates that each precipitated band of D depletes the surrounding gel of B by acting as a sink and thus creates a zone spared by the precipitation front by lowering [AB] under q* [24].

Each precipitated DNA pellet was re-suspended in 25 μl of 1 mM Tris (pH 8.0).

Genomic DNA of each precipitated strain was extracted with a bacterial genomic DNA extraction kit (BioColor Inc., Shanghai, China) and then dissolved in TE buffer for detecting the DNA concentration with ultraviolet (UV) spectrophotometry [ 37].

Each precipitated protein pellet was re-dissolved in 50 μl of reducing Laemmli buffer (with 10 mM DTT) for 1 h at 56 °C and alkylated with 1% acrylamide at room temperature for 30 min for subsequent GeLC-MS/MS analysis.

To determine the purity of the fractions, 250 µl of each was precipitated with 20% (w/v) Tri-chloroacetic acid (TCA).

Samples of two selected fractions (100 µg of total protein each) were precipitated with acetone at −20°C for 2 hours and then pelleted at 13,000× g for 15 min. The proteins were trypsin digested and labeled with sample-specific iTRAQ reagents according to the manufacturer's protocol (Applied Biosystems).

Western blot analysis was then performed using eIF4G- and hHpr1-specific antibodies to assess the relative amounts of each protein precipitated by ORF57 in the absence or presence of PYMΔNΔC-FLAG.

After quantification, 100 µg of total proteins from each condition was precipitated using six volumes of cold acetone to remove interfering substances.

The proteins in each pool were precipitated by methanol and labeled with Cy3 and Cy5, respectively, and then combined for 2D-DIGE.

For Western blot analysis, 700 µg of proteins from each sample were precipitated twice in cold acetone 80% and 0.2 mM DTT for 30 minutes at 16,000 g and 4°C.

A total of 900 µg of proteins from each sample was precipitated with cold acetone and resuspended in thiourea buffer (7 M urea, 2 M thiourea 2% CHAPS, 2% ASB-14, 5% glycerol, 40 mM DTT, 4 mM TCEP, 1% 3 10 IPG buffer, Amersham), and the samples were mixed overnight at 4°C in the dark and clarified by centrifugation at 16000 g for 15 minutes at 4°C.