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Molecular markers flanking the most significant QTL in each population were placed on the physical map of lettuce19.
Thirty individuals, randomly sampled from each population were genotyped at five SSR loci and fingerprinted using seven AFLP primer combinations.
Five representative individuals from each population were collected as herbarium vouchers and deposited at the herbarium of the Natural History Museum, University of Oslo (O).
Molecular markers flanking the most significant QTL in each population were placed on the physical map of lettuce (version 8) assembled for cv.
The means and standard deviation of each population were averaged across triplicate measures.
Five individuals (n = 5) from each population were sampled over the entire population area at the flowering stage (November 2012).
At least five mature leaves in each population were fixed in a mixture of 70% ethyl alcohol and 0.05% glycerol.
Three independent tests corresponding to each population were run sequentially.
All fish from each population were from a single family.
Two voucher specimens for each population were deposited in the Herbario Nacional de México (MEXU).
A slightly less conservative estimate indicated that 5% of colonies in each population were chimeras.
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