Sentence examples for each plasmid were from inspiring English sources

For short-term stability study, 3 bottles of each plasmid were placed at 4 °C, for different storage time (0, 1, 3, 7 and 15 days) and quantified by UV, then the analysis data was analysed by the classic linear model and t test.

DNA sequences of each plasmid were confirmed.

Before doing the experiment, 100 ng of each plasmid were loaded into an agarose gel for mass standardization.

The nucleotide sequences of each plasmid were analyzed using VectorNTI version 10.3 (Invitrogen, Carlsbad, CA) and a rare cutting restriction enzyme that cleaves the plasmid once was selected.

Each plasmid was injected separately and subsequent injections of each plasmid were into the same muscle: PkCSP right rectus femoris, PkSSP2 left rectus femoris, PkAMA1 right triceps, and PkMSP1 left triceps.

E. coli strain BL 21 (DE 3) cells carrying each plasmid were grown in 2xTY culture containing 100 µg/ml of ampicillin and 1.0 mM 5-aminolevulinic acid (Wako Chemicals, Osaka, Japan) at 37C.

CYP2E1 activity in Salmonella typhimurium cells transformed with each plasmid was assessed by mutagenic reversion frequency in the presence of dimethylnitrosamine.

Each plasmid was transformed into R. leguminosarum strains 1062, RES-2 and RES-9 to generate nine donor plasmid combinations, and their mobilisation frequencies into Escherichia coli and Agrobacterium tumefaciens measured following biparental matings.

Each plasmid was introduced into MH7A cells, and protein expression was confirmed by western blot analysis.

Each plasmid was then transformed into E. coli BL21 (DE3) harboring pRARE (Table 1).

Each plasmid is included in pCR21 vector (Invitrogen) supplied by L.M. Steinberg and J.M. Regan, Pennsylvania State University.