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Each plasmid was then transformed into E. coli BL21 (DE3) harboring pRARE (Table 1).

The luciferase activity of each plasmid was shown in B. (C) A dual luciferase assay.

Each plasmid was introduced into MH7A cells, and protein expression was confirmed by western blot analysis.

CYP2E1 activity in Salmonella typhimurium cells transformed with each plasmid was assessed by mutagenic reversion frequency in the presence of dimethylnitrosamine.

Each plasmid was transformed into R. leguminosarum strains 1062, RES-2 and RES-9 to generate nine donor plasmid combinations, and their mobilisation frequencies into Escherichia coli and Agrobacterium tumefaciens measured following biparental matings.

One bottle of each plasmid was randomly taken out from the batch and analysed for 3 times (n = 3) by UV at 0.5, 1, 2, 4, 6, 9 and 12 months.

Each plasmid was transformed by electroporation into E. coli S17-1, wasch was then used as a donor strain for the conjugative transfer of plasmid into R. eutropha by a standard mating procedure [24].

Each plasmid was sequenced.

Each plasmid was transfected into COS1 cells.

A 150 ml culture of E.coli bearing each plasmid was divided into three 50 ml parts.

Copy number of each plasmid was calculated by the iQ5 optical system software.

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