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The settings for each option of the program were the same as the default options presented previously [ 1], with the exception of the RIP program, which was executed with a window size of 200 and a step size of 50, to compensate for the low divergence rates in the data sets.
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We then built a searchable database of this genome and BLASTed the consensus sequences of each GBS-seq and microsatellite locus to this database using the 'blastn' option of the program blast v2.2.25+ [ 66].
In order to effectively model genotype by environment interaction (GxE, with environments represented by the different treatments) through QTL by environment interaction (QTLxE), each trait was analysed individually using the single trait - multiple environment option of the program.
The haplotype phases of both parents in AF and US family-trios were estimated based on the genotype data of the corresponding offspring using the P1 option of the program.
The remaining residues for which no structural information is available were generated using the loop refinement option of the program Modeller 8.0 [70], [70] while keeping the TM domain spatially constrained.
The segregation data of all populations were arranged according to the consensus order in separate Excel spreadsheets and graphical genotypes were displayed in color using the conditional formatting option of the program.
COSY and HMBC spectra can, for certain physical reasons [33, 34], also exhibit signals corresponding to the coupling of spins separated by distances larger than the default options of the program.
Input options of the program include mapping of a single peptide or a set of peptides on the antigen structure, and the results of the calculation can be visualized on our interactive web server.
Better yet, both products give you the option of installing the program and all the data on your PC's hard drive.
To reinforce training, counselors were provided with a worksheet outlining intervention procedures, and they were also given the option of shadowing the program coordinator.
The teenagers' parents had previously been informed by the school about the peer-led sex education program, and they were given the option of refusing the program; in the which case, the pupil went to study in another room.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com