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Resident and migratory individuals were sampled from each of two populations: Sashin Creek, Alaska and Little Sheep Creek, Oregon.
A QTL for resistance was revealed in each of two populations derived from crosses between a resistant accession and two different susceptible accessions.
Using silica-dried leaf tissue from one plant from each of two populations (Appendix 1), total DNA was isolated using cetyltrimethylammonium bromide (CTAB) extraction (Doyle and Doyle, 1987).
Four males (size-matched within 5 g) and 10 15 females of each of two populations were present throughout the experiment.
Formally, let p i be the frequency of a variant in biallelic SNP in each of two populations (i = 1,2).
Applying the method of Oleksyk et al. [ 19], three values were calculated for each window: median multilocus heterozygosity for each of two populations and the multilocus variance of F ST between them.
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The relative migration of two populations in each of the tissues was expressed as a ratio of the radioactivity of intestinal/subcutaneous lymphocytes recovered from that tissue.
For C. forbesii, 20 samples were collected randomly from each of five populations from the Baja California mainland (Figure 1).
After each mtDNA was assigned to the most-derived named haplogroup, the haplogroup distribution frequencies in each of seven populations were estimated.
Sequencing was done both individually for each isofemale strain, as well as in sequenced pools for each of three populations.
Using two individuals from each of three populations, whole-body mRNA was normalized and sequenced (110Mb total).
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CEO of Professional Science Editing for Scientists @ prosciediting.com