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RFLP assays were designed to detect each of the variant CYP1A1 alleles.
PCR-RFLP assays were designed to detect each of the variant CYP1A1 alleles.
Each of the variant alleles led to significantly decreased luciferase activity.
Also, an additive genetic score was created from the two polymorphisms in which the presence of each of the variant alleles for each polymorphism was scored as one point.
PONDR VL-XT disorder predictions (www.molecularkinetics.com) (15) were carried out on full-length wild-type and each mutated protein sequence, generated by changing each of the variant residues individually.
To assess a potential violation of the first assumption (i.e., a true association between each of the variant and TL), an additional analysis was performed after excluding the SNP near the PXK region (rs6772228), which may be a false-positive association evidenced by its lack of plausible biological explanation, and the lack of consistency in its association with TL across several study sites (21).
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Each of the variants was incubated with 20 mM 3-HPA for the indicated periods, and the residual activity at the end of the incubation was measured.
Each of the variants comprises different transportation solutions and concepts as well as distinct investments in transportation infrastructure.
The details of crystallographic data gained for each of the variants are summarized in Supplementary Table 4. Wild-type and SDM variants of HydA1 and CpI were probed by in situ ATR-FTIR spectroscopy29,34,57 using a rapid-scan Tensor 27 spectrometer (Bruker Optik, Germany) equipped with a three-reflection ZnSe/silicon crystal ATR cell (Smith Detection, USA).
All of the key performance parameters for each of the variants have been satisfied, and the plane is flying every day (there have been over 13,000 operational sorties).
In the no selection model (simulation A), there is no selective pressure because each of the variants has a 50% chance of winning the competition event.
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