Sentence examples for each of the transformants from inspiring English sources
Southern blot analysis suggested that 87.5 % of transformants obtained with this protocol displayed single hybridization bands, indicating a single insert of T-DNA in each of the transformants.
For confirmation of the suspected transcription, total RNA samples were extracted from the flowers of each of the transformants, reverse-transcribed, and amplified with primer pairs Atgcs1-seqA2 and GFPBDr, and GFPBDf and Atgcs1truer (primer sets A and B in Figure 1C, respectively).
Wild-type A. oryzae and each of the transformants were cultivated in GPY medium and harvested by filtration with Miracloth (Calbiochem, Darmstadt, Germany).
Two leaves per plant from three copies of each of the transformants were infiltrated with the following constructs: two effectors (Avrvnt1 and IpiO = Avrsto1) [ 13, 24], R3a[ 38] and Avr3a[ 39] as the positive control and empty pK7WG2 [ 40] as the negative control.
Functional (floating) recombinant gas vesicles were readily isolated from each of the transformants and then tested to detect recombinant GvpC expressing the SIVTat, Rev or Nef1 encoded peptide and assessed for recognition as SIV genome derived using sera from an SHIV infected monkey.
As shown in Figure 5, the asterisk indicates that the different silenced genes occurred simultaneously in each of the transformant lines.
Based on this expected scenario, wild type females were pollinated with pollen from each of the mAtGCS1 transformants, and the resulting offspring seeds were sown in Kan- and Gen-containing media to assess which construct complements the GCS1 mutation (Figure 3B).
For each of these vectors, 100% of the transformants were coloured.
A majority of the transformants resistant to the antibiotic G418 were capable of synthesizing and secreting biologically active human interferon.
Next, we analyzed the growth of the transformants in liquid media using a Cell Growth Quantifier (Aquila Biolabs)39.
The growth impairment of the transformants due to the presence of pheS* was verified on cPhe-containing agar plates followed by verification through Sanger sequencing.
