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Fermentative, photosynthetic, and in vitro hydrogenase activities were assayed in each of the mutant genotypes.
Each of the mutant genotypes is stable on vegetative transfer and retains its characteristics without recombination in hybrids, thus establishing each mutant as a genuine allele5.
Each of the mutant neo constructs was introduced into the packaging helper cell line psi 2 by sequential cotransfection, and individual psi 2 double transformants were selected.
Note that only Cu but not Fe accumulation has changed in each of the mutant lines and that Cu was barely detectable in the double mutant.
The reductions in adhesion or invasion that were observed in each of the mutant strains are represented as percentages.
First, they subjected each of the mutant strains to low-oxygen conditions and identified 28 genes whose absence stopped the yeast from growing.
A total of at least two clones of each of the mutant lines were picked and screened for phenotypic characterization.
Each of the mutant strains used in this work were created using allelic exchange, resulting in markerless deletions (Figure 1).
Individuals with the αB-crystallin arginine 120 to glycine (R120G) mutation typically have one copy each of the mutant and wild-type allele.
Repeated attempts to create a ΔiPGM, ΔdPGM double deletion by targeting the remaining locus in each of the mutant strains were unsuccessful.
Pre-implantation embryos do not provide sufficient material for Southern analysis and PCR genotyping cannot distinguish homozygous embryos for each of the mutant alleles individually from heterozygous embryos.
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